Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Trp63

Cell type

Cell type Class
Lung
Cell type
Lung
MeSH Description
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.

Attributes by original data submitter

Sample

source_name
ΔNp63fl/fl and ΔNp63Δ/Δ mice
tissue
lung
cell type
AT2 cells
genotype
DNp63 WT
chip antibody
P63 (Biolegend, 619002)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell pellets were spun down and flash frozen. Samples were sent on dry ice to Active Motif where they used their proprietary low-cell number ChIP-seq extraction protocol. Sequencing librairies were prepared using Bioo Kit Option 2 protocol using 1.7-10 ng of DNA per sample.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
49104901
Reads aligned (%)
48.4
Duplicates removed (%)
56.4
Number of peaks
849 (qval < 1E-05)

mm9

Number of total reads
49104901
Reads aligned (%)
48.3
Duplicates removed (%)
56.5
Number of peaks
789 (qval < 1E-05)

Base call quality data from DBCLS SRA