Antigen

Antigen Class

TFs and others

Antigen

Trp63

Cell type

Cell type Class

Lung

Cell type

Lung

MeSH Description

Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.

Sample

source_name

ΔNp63fl/fl and ΔNp63Δ/Δ mice

tissue

lung

cell type

AT2 cells

genotype

DNp63 WT

chip antibody

P63 (Biolegend, 619002)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell pellets were spun down and flash frozen. Samples were sent on dry ice to Active Motif where they used their proprietary low-cell number ChIP-seq extraction protocol. Sequencing librairies were prepared using Bioo Kit Option 2 protocol using 1.7-10 ng of DNA per sample.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

49104901

Reads aligned (%)

48.4

Duplicates removed (%)

56.4

Number of peaks

849 (qval < 1E-05)

mm9

Number of total reads

49104901

Reads aligned (%)

48.3

Duplicates removed (%)

56.5

Number of peaks

789 (qval < 1E-05)